Setting up Pi-Hole on a Virgin Vincent modem-router

Yes.
A DHCP reservation from the router wont work anymore once you've disabled the router DHCP service (the source of the reservation).
You need to configure the Pi with a true static IP for it to be able to function as a DHCP server for your LAN.
For Pi-OS Bookworm you can use the nmtui tool for that (Network Manager Text User Interface):

sudo nmtui

Example below:

Yes you flip on the router DHCP service again.